Incubation is the act Forced draft incubators
  I. Based on heating  source:
II. Based on fuel used
Location
  The chick  hatcheries are modern buildings that provide separate rooms for each hatchery  operations, but each room has its individual requirements.  The hatchery area should be a separate unit  with its own entrance and exit, unassociated with those of the poultry farm.  The hatchery should be situated at least 1000 ft from poultry houses to prevent  horizontal transmission of disease-producing organisms from the chicken houses  to the hatchery.
  Size  of the hatchery
  The size of the  hatchery is based on the egg capacity of the setters and hatchers, number of  eggs that can be set each week and number of chicks hatched each week.  Also, necessary space to be allotted for  future expansion.
  Hatchery  design
  Hatchery should be  constructed in such a manner that the hatching eggs may be taken in one end and  the chicks removed at the other.  In  other words, eggs and chicks should flow through the hatchery from one room to  the one next needed in the hatching process.   There should not be no backtracking.   Such a flow affords better isolation of the rooms and there is less  human traffic throughout the building.
  Hatchery  construction
  Hatchery buildings  should be intricately designed, properly constructed, and adequately  ventilated.  Brief general points to be  considered are,
Hatchery rooms/ structures
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  Hatchery rooms must  be adequate in size.  Usually, hatcheries  of medium size will hatch chicks twice a week, but large hatcheries will hatch  more than two hatches per week.  Consequently,  hatching schedules will affect the size of some rooms in the hatchery.  
  Shower  room
  To maintain  bio-security it is essential that all persons entering the premises shower and  change into clean clothing in an adjoining room.  It is the only entrance and exit, and the  hatchery becomes an isolated unit as far as human beings are concerned.
  Hatching  eggs receiving counter
  Employees  delivering hatching eggs to the hatchery must not enter the hatchery in the  course of their duties.  Eggs should be  delivered to the hatchery through a specialized door.
  Fumigation  room
  The fumigation room  should be as small as possible in order to reduce the amount of fumigant  used.  A fan should be used to circulate  the air and exhaust the fumigant.
  Egg  holding (egg-cooler) room
                                 
  Egg holding room  should be about 8 ft high, insulated, slowly ventilated, with complete air  movement, cooled, and humidified.  The  room must be refrigerated to maintain a temperature of 650c  A forced-air type of refrigeration unit is  required in order to keep a uniform temperature throughout the room.
  Pre  incubation warming room
  Here eggs are kept for the purpose of drying the ‘sweat’ over eggs.  It can be achieved by providing sufficient  number of ceiling fans in this room.
  Setter room
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  Setters  (incubators) are kept in this room.  The  size of the setter room will depend on the make of the equipment used.  The incubating equipment takes relatively  little floor space.  The exact room size  involves the aisle and working area necessary to move the eggs and chicks in  and out of the machines.  A minimum space  of 3 ft should be allotted between the sides of adjacent setters and from wall  to sides or back of the setters.   Similarly, minimum of 10 ft should be allotted in front of two setters  when kept face-to-face arrangements.
  Egg  candling (dark) room
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This room is usually constructed in between setter and hatcher room for candling eggs. Candling is usually practiced when eggs are transferred from setter to hatcher. Provisions should be made to dark the room to facilitate easy candling.
  Hatcher  room
  Hatchers are kept  in this room.  Here sufficient spaces are  to be allowed around hatcher similar to that of setter room.  Since it is prone for contamination with  fluffs and debris at the time of hatching, the door towards setter room is to  be tightly closed unless the necessity arises (at the time of egg transfer).
  Chick  holding room
  Next to hatcher  room, chick-holding room is present.  A  relative humidity of 65% is maintained to prevent excessive chick  dehydration.  Here, the chicks are  sex-separated, graded, vaccinated and placed in chick boxes.
  Wash  room
  After chicks are  boxed, the trays are washed in a tray washer in the washroom.  Necessary pipelines with high-pressure pumps  are kept in this room.
  Clean  room
  After the trays are  washed, they are placed in their trolleys and moved to the adjacent clean room.
Five major functions are involved in the incubation and hatching of poultry eggs. They are:
1. Temperature
        Temperature  is the most critical environmental concern during incubation because the  developing embryo can only withstand small fluctuations during the period.  Embryo starts developing when the temperature exceeds the Physiological Zero.  Physiological  zero is the temperature below which embryonic growth is arrested and  above which it is reinitiated.  The  physiological zero for chicken eggs is about 75oF (24oC).  
        The optimum  temperature for chicken egg in the setter (for first 18 days) ranges from 99.50 to 99.75 o F and in  the hatcher (last 3 days) is 98.50  F.
        2. Humidity
        Incubation  humidity determines the rate of moisture loss from eggs during incubation.  In general, the humidity is recorded as  relative humidity by comparing the temperatures recorded by wet-bulb and  dry-bulb thermometers. 
        Recommended  incubation relative humidity for the first 18 days ranging between 55 and 60% (in setter) and for the last  3 days ranging between 
        65 and 75%.  Higher humidity during hatching period is  given to avoid dehydration of chicks.
        3. Ventilation
        Ventilation  is important in incubators and hatchers because fresh oxygenated air is needed  for the respiration (oxygen intake and carbon dioxide given off) of developing  embryos from egg setting until chick removal from the incubator. 
        The oxygen needs  are small during the first few days compared to the latter stages of  development. Oxygen content of the air at sea level is about 21%.  Generally the oxygen content of the air in  the setter remains at about 21%.  For  every 1% drop in oxygen there is 5% reduction in hatchability.  
        Carbon dioxide is a  natural by-product of metabolic processes during embryonic development and is  released through the shell.  The tolerance level of CO2 for  the first 4 days in the setter is 0.3%. CO2 levels above 0.5% in the  setter reduce hatchability and completely lethal at 5.0%.
        Since the normal  oxygen and CO2 concentrations present in air seem to represent an  optimum gaseous environment for incubating eggs, no special provision to  control these gases is necessary other than to maintain adequate circulation of  fresh air at the proper temperature and humidity.
        4. Position of eggs
        Artificially  incubating eggs should be held with their large ends up.  It is natural for the head of the chick to  develop in the large end of the egg near the air cell, and for the developing  embryo to orient itself so that the head is uppermost.  When the eggs are incubated with the small  end up, about 60% of the embryos will develop with the head near the small  end.  Thus, when the chick is ready to  hatch, its beak cannot break into the air cell to initiate pulmonary  respiration.  Eggs positioned  horizontally will incubate and hatch normally as long as they are turned  frequently. Under normal circumstances eggs are set with large end up for the  first 18 days (in setter) and in horizontal position for the last 3 days (in  hatcher).
        5. Turning of eggs
        Birds,  including chickens and quail, turn their eggs during nest incubation. Nature  provides nesting birds with the instinct of turning eggs during  incubation.  Similarly eggs to be turned  at least 8 times a day. Turning of  eggs during incubation prevents the developing  embryo adhering to the extra-embryonic membranes and reduces the possibility of  embryo mortality. In large commercial incubators the eggs are turned  automatically each hour i.e. 24 times a day. Most eggs are turned to a position  of 45o from vertical, and then reversed in the opposite direction to  45o from vertical.  Rotation  less than 45o are not adequate to achieve high hatchability. Turning is not required in Hatcher.
Factors  | 
            Setter  | 
            Hatcher  | 
          
Temperature  | 
            99.50 to 99.75 o F  | 
            98.5 o F  | 
          
Relative Humidity  | 
            55-60 %  | 
            65-70 %  | 
          
Position  | 
            Large end up  | 
            Horizontal  | 
          
Turning  | 
            Manual - 8 times  | 
            No turning  | 
          
        Handling  of Hatching Eggs and Storage
The quality of  hatching egg cannot be improved after lay but one can reduce the loss in  hatching egg quality by adopting some standard procedures.  
Maintaining egg quality in  the breeder house
        The  hen will lay her eggs over nesting material.   Use of enough clean, dry and mold-free nesting material can avoid  cracked and dirty eggs. Similarly hens to be trained to use nests to lay eggs  instead of laying on floors by providing sufficient number of nest boxes well  in advance before the laying starts.             
        The  frequency of hatching egg collection is very important to maintain  quality.  Hatching eggs should be  collected at least 4 times a day. Hatching eggs are susceptible to  contamination and every effort must be made to reduce the microbial load.  Therefore, it is imperative that people wash  and sanitize their hands before collecting eggs from the nests or egg  belts.  The flats that eggs are placed on  must also be sanitized and free of organic material.
      
Selection of hatching eggs
          Not all eggs laid by a breeding flock are  set.  Eggs that are cracked, dirty or  misshapen are usually not used for hatching.   Very small or very large eggs do not hatch as well as eggs in the middle  size range.  Eggs with thin or very  porous shells are not likely to hatch well because of excessive losses of water  during incubation.
  
  Reducing  contamination of hatching eggs
          Poor hatching egg sanitation can be a major  cause of lower hatchability and poor chick quality.  There is no such thing as a sterile  eggshell.  More bacteria are picked up on  the shell when the egg passes through the cloaca where urine and intestinal  contents also pass.  The bacterial load  found on an eggshell at the time of lay ranges from 300 to 500 organisms.  After oviposition, every surface the egg  comes in contact with can further inoculate the shell surface.  After an egg is laid it begins to cool.  During the cooling process the egg contents  begin to shrink and producing negative pressure.  This is one of the more opportune times for  bacteria on the shell surface to penetrate the eggshell.
        Egg  has many natural defense mechanisms to reduce bacterial penetration.  The shell itself provides some  protection.  The cuticle on the surface  of the eggshell is the best natural barrier to penetration.  The inner and outer shell membranes provide  additional barriers. The albumen provides a somewhat effective control over  contamination.  The albumen has a high pH  in which most bacteria cannot survive.   The chalazae contain an enzyme, lysozyme, which has antibacterial  properties.
        Many  breeder people choose some methods to reduce the microbial load over the  eggshell.  Sanding, buffing, and wiping  the hatching eggs are not good methods of sanitation.  Sanding and buffing will remove at least part  of the cuticle resulting in eggs that are more susceptible to penetration.  Fumigation with formaldehyde gas is an  effective method of sanitizing hatching eggs.    Solutions containing quaternary ammonium compounds, formalin, hydrogen  peroxide or phenols may be moderately effective in reducing the microbial load  over hatching eggs. DO NOT wash eggs unless necessary. If it is necessary to  wash eggs always use a damp cloth with water warmer than the egg. This causes  the egg to sweat the dirt out of the pores. Never use water cooler than the  egg. Also, do not soak the eggs in water. 
        
       Storage  of hatching eggs
In normal hatchery operations, eggs cannot be set immediately after they are laid. Many hatcheries set eggs once or twice in a week. If hatching eggs are stored up to 1 week, hatching eggs should be kept in an egg holding room with the temperature of 65oF and the relative humidity of 75%. When storing eggs less than 10 days, store them with the large end up. If eggs are held for 10 days or more, hatchability will be improved if stored with small end up.
        The operation of a chick hatchery involves the  production of the largest number of quality chicks possible from the hatching  eggs received at the hatchery.  In  addition, chicks must be produced economically. The sequences of hatchery operations followed in  commercial hatcheries are
        Advantages
        
1) Securing hatching eggs
                  Hatcheries can get the hatching eggs from  any one of the following ways:
2) Traying hatch eggs
      
Traying hatch eggs  | 
        
        
                  The eggs from the breeder flocks should be  transferred to the egg setter trays in the hatchery immediately after  receiving.
        3) Fumigation of hatching  eggs
                  After traying, eggs are to be kept in the  fumigation chamber for fumigation. Fumigating with 3x concentration of  formaldehyde for 20 minutes will kill about 97.5 to 99.5% of the organisms on  the shells.  One ‘x’ concentration means  20 g of KMnO4 with 40 ml of formalin for 100 cubic feet (3x means 60 g of KmnO4  + 120 ml of formalin for 100 cubic feet).
        4) Cold Storage
                  When the eggs are not set immediately after  receiving, they should be kept in cold rook at the temperature of 65 oF  and 75% relative humidity.
        5) Warm eggs prior to  setting
                  Approximately 6 hours prior to placing eggs  in the setter they should be moved from the egg-cooler room to normal room  temperature.  Here, atmospheric air  condenses over eggshell and form water droplets over eggshell, which is called  as ‘Sweating’. It is advantageous to warm eggs before placing them in  the incubator by avoiding creation of low temperature in the machine by placing  cool eggs directly.
        6) Loading of eggs
                  Placing of eggs in the setter is called ‘Loading  of eggs’.  Eggs can be set in the  setter either all-in all-out basis or batch basis.  Most of the commercial hatcheries are  practicing batch system of loading eggs in the setter that will minimize the  initial time taken to reach normal incubation temperature in the setter.  In this case, each setter is having hatching  eggs with different stages of embryonic developments. 
        7)  Candling
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        Candling is a  process in which eggs are kept in front of a light source to find out the  defects in eggshell, embryonic development etc. Candling can be done as early  as five days of incubation, but errors in candling often occur at this time.  Under commercial operations, candling is done when the eggs are transferred  from setter to hatcher (at 19th day for chicken eggs).  There are two methods of candling that can be  used. The fastest method involves the use of a table or mass candler. An  entire tray of hatching eggs may be placed on the mass candler and examined  with one observation. Candling with a spot candler or individual candler  is a little slower, but it is more accurate.
        8) Transfer of eggs
                  In modern incubators, eggs are transferred  from setter to hatcher at 19th day of incubation (for chicken egg) or when  approximately 1% of the eggs are slightly pipped. In general, one-seventh of  total incubation period is needed to keep eggs in the hatcher. 
        9) Pulling the hatch
                  The process of removing the chicks from the  hatcher is often called pulling the hatch.  Chicks should be removed from the hatcher as soon as all are hatched  and about 95% are dry.  Excessive drying in  the hatcher should be avoided.
        10) Hardening the chicks
                  When the chicks are first placed in the  chick boxes they are soft in the abdomen, are not completely fluffed out, and  do not stand well.  They must be  “hardened” by leaving them in the boxes for 4 or 5 hours.  Such hardening makes it easier to grade the  chicks for quality, and the chicks are more easily vent-sexed.
        11) Grading the chicks
                  No chick below the minimum standard must be  allowed to go to a customer.  Some  standards for quality are, 1) No chick deformities 2) No unhealed navels 3)  Above a minimum weight 4) Not dehydrated and 5) Stand up well.
        12) Sexing the chicks
      
Sexing the chicks  | 
        
        
                  Layer type day-old chicks are need to be  sex separated either by vent sexing or auto-sexing (feather sexing). In case of  meat-type day-old chicks sexing is not practiced.
        13) Vaccination
      
Vaccination  | 
        
        
                  Most chicks are vaccinated against Marek’s  disease in hatchery before delivery. Most common method of vaccination of  day-old chicks is by subcutaneous method in the nape of the neck.  
        14) Chick delivery
                  Baby chicks should reach the customer’s  farm early in the morning.  Not only the  weather is cooler during this part of the day but the early arrival allows a  full day for close observation of the chicks by the caretaker. 
        15) Washing and cleaning
      
Washing and cleaning  | 
        
        
                  Cleaning the hatchery between hatches is of  primary importance.  The process must be  complete.  Except for the setters and  setter room, every piece of equipment must be thoroughly vacuumed, scrubbed,  disinfected and fumigated.
        16) Disposal of waste
      
Disposal of waste  | 
        
        
                  Hatchery wastes include infertile and  non-hatched eggs, and dead and cull chicks that should be disposed in such a  manner not to create problem to the neighbors and also not to contaminate the  hatchery premises. 
        
        Steps Involved  In Commercial Hatchery Operations
                           